| First Author | Spahn TW | Year | 2002 |
| Journal | Eur J Immunol | Volume | 32 |
| Issue | 4 | Pages | 1109-13 |
| PubMed ID | 11920578 | Mgi Jnum | J:75996 |
| Mgi Id | MGI:2178191 | Doi | 10.1002/1521-4141(200204)32:4<1109::AID-IMMU1109>3.0.CO;2-K |
| Citation | Spahn TW, et al. (2002) Mesenteric lymph nodes are critical for the induction of high-dose oral tolerance in the absence of Peyer's patches. Eur J Immunol 32(4):1109-13 |
| abstractText | We have previously demonstrated the loss of oral tolerance (OT) in lymphotoxinalpha - / - (LTalpha - / -) and TNFalpha / lymphotoxinalpha deficient (TNFalpha / LTalpha - / -) mice which have defective Peyer's patches (PP) and lymph node (LN) development. We have now studied OT in BALB / c mice with differential defects of the gut-associated lymphoid tissue (GALT) caused by inhibition of LTbetaR signaling during fetal development. Treatment of pregnant mice with LTbetaR-IgG (LTbetaRIgG) and TNFR I(55)-IgG (TNFR55IgG) abrogates the formation of PP (LTbetaRIgG) or of PP and mesenteric LN (MLN) (LTbetaRIgG / TNFRIgG) without genetically deleting the respective cytokine pathways. OT was readily induced in mice without PP but retaining MLN (PP null / LN +). In contrast, OTcould not be induced in mice lacking both MLN and PP (PP null / MLN null) as shown by the inability of these mice to suppress IFN-gamma secretion or DTH reactions. We next assessed OT in 129 x B6 LTalpha - / - mice with and without MLN. Timed treatment of pregnant LTalpha - / - mice with an agonist anti-LTbetaR mAb induces formation of MLN but not of PP in LTalpha |
