| First Author | Snapper CM | Year | 1996 |
| Journal | J Immunol | Volume | 156 |
| Issue | 1 | Pages | 183-91 |
| PubMed ID | 8598461 | Mgi Jnum | J:30268 |
| Mgi Id | MGI:77782 | Doi | 10.4049/jimmunol.156.1.183 |
| Citation | Snapper CM, et al. (1996) B cells from p50/NF-kappa B knockout mice have selective defects in proliferation, differentiation, germ-line CH transcription, and Ig class switching. J Immunol 156(1):183-91 |
| abstractText | To better understand the role of NF-kB in normal B cell physiology, we used a purified population of resting B cells from p50/NF-kappa B knockout (p50-/-) mice to determine their ability to proliferate, secrete lg, express germ-line CH, RNA, and undergo lg isotype switching in vitro in response to a number of distinct stimuli. p50-/- B cells proliferated normally in response to dextran-anti-IgD Abs (alpha delta-dex) and membrane-bound, but not soluble, CD40 ligand (CD40), and they were virtually unresponsive to LPS when compared with control B cells. p50-/- B cells secreted markedly reduced lg in response to alpha delta-dex or mCD40L in the presence of IL-4 + IL-5, despite their relatively normal proliferative rates, whereas normal lg secretion was restored by the combination of alpha delta-dex and CD40L. p50-/- B cells expressed normal steady-state levels of germ-line CH gamma 3 and CH gamma epsilon RNA upon appropriate stimulation. Although p50-/- B cells underwent substantial switching to IgG1, a marked reduction in the switch to IgG3 and IgE, as IgA, was observed. These data are the first to demonstrate key, independent roles for |
