| First Author | Peterson JC | Year | 2018 |
| Journal | Dis Model Mech | Volume | 11 |
| Issue | 10 | PubMed ID | 30242109 |
| Mgi Jnum | J:266206 | Mgi Id | MGI:6203295 |
| Doi | 10.1242/dmm.034637 | Citation | Peterson JC, et al. (2018) Nos3 mutation leads to abnormal neural crest cell and second heart field lineage patterning in bicuspid aortic valve formation. Dis Model Mech :dmm034637 |
| abstractText | The bicuspid aortic valve (BAV), a valve with two instead of three aortic leaflets, belongs to the most prevalent congenital heart diseases in the world occurring in 0.5-2% of the general population. We aimed to understand how changes in early cellular contributions result in BAV and impact cardiovascular outflow tract development.Detailed 3D reconstructions, immunohistochemistry and morphometrics determined that during valvulogenesis the non-coronary leaflet separates from the parietal outflow tract cushion instead of originating from an intercalated cushion.Nos3(-/-) mice develop a BAV without a raphe as a result of incomplete separation of the parietal outflow tract cushion into the right and non-coronary leaflet.Genetic lineage tracing of endothelial, second heart field and neural crest cells revealed altered deposition of neural crest cells and second heart field cells within the parietal outflow tract cushion of Nos3(-/-) embryos. The abnormal cell lineage distributions also affected the positioning of the aortic and pulmonary valves at the orifice level.The results demonstrate that the development of the right and non-coronary leaflets are closely related. A small deviation in the distribution of neural crest and second heart field populations affects normal valve formation and results in the predominant right-non type BAV in Nos3(-/-) mice. |
