| First Author | Salmond RJ | Year | 2008 |
| Journal | Int Immunol | Volume | 20 |
| Issue | 7 | Pages | 819-27 |
| PubMed ID | 18448457 | Mgi Jnum | J:136717 |
| Mgi Id | MGI:3796816 | Doi | 10.1093/intimm/dxn040 |
| Citation | Salmond RJ, et al. (2008) CD4+ T cell hyper-responsiveness in CD45 transgenic mice is independent of isoform. Int Immunol 20(7):819-27 |
| abstractText | The CD45 tyrosine phosphatase is required for T cell development and function by virtue of its role as a positive regulator of src family kinase activity. In addition, recent data have highlighted that CD45 also acts as a negative regulator of Lck function by dephosphorylation of critical tyrosine residues. Lck functionality and TCR responsiveness are elevated in transgenic mice expressing the CD45RO isoform at 'intermediate' (10-40% of wild type) levels, indicating that the expression level of CD45 is critical in determining the sensitivity of T cells to TCR stimulation. However, it is unclear whether such a phenotype is specific for the CD45RO isoform, typically expressed by activated T cells. In the present work, the roles of three isoforms of CD45, RO, RB and RABC, in thymocyte development, T cell responses and TCR signalling pathways were directly compared. The data demonstrate that expression of CD45RB or CD45RABC at intermediate levels also results in CD4(+) T cell hyper-reactivity, as previously published for CD45RO. These data emphasize the dual functions of CD45 as both a positive and a negative regulators of TCR signalling irrespective of specific isoform expression. |
