| First Author | van Oers NS | Year | 1994 |
| Journal | Immunity | Volume | 1 |
| Issue | 8 | Pages | 675-85 |
| PubMed ID | 7600293 | Mgi Jnum | J:189423 |
| Mgi Id | MGI:5445500 | Doi | 10.1016/1074-7613(94)90038-8 |
| Citation | van Oers NS, et al. (1994) ZAP-70 is constitutively associated with tyrosine-phosphorylated TCR zeta in murine thymocytes and lymph node T cells. Immunity 1(8):675-85 |
| abstractText | Studies with T cell lines and clones have shown that engagement of the TCR results in the tyrosine phosphorylation of the TCR subunits. This leads to the recruitment of the ZAP-70 protein tyrosine kinase, an interaction involving the two SH2-domains of ZAP-70 with tyrosine-phosphorylated zeta and CD3. However, as previously described, murine thymocytes and lymph node T cells express a constitutively tyrosine-phosphorylated zeta subunit in the basal state. Here, we show that a fraction of ZAP-70 molecules are constitutively associated with tyrosine-phosphorylated zeta. TCR ligation promotes a large increase in the tyrosine phosphorylation of ZAP-70 as well as other TCR subunits. Genetic studies reveal that the constitutive ZAP-70 association with tyrosine-phosphorylated zeta does not absolutely require either TCR or coreceptor interactions with MHC molecules. |
