Primary Identifier | MGI:6719375 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Calcr |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A targeting plasmid containing the porcine teschoviral 2A cleavage sequence linked to Cre recombinase and a Frt-flanked neomycin resistance gene replacing the stop codon of the Calcr gene in a bacterial artificial chromosome (RP24-193M22) was used for embryonic stem cell targeting. Chimeric animals generated were mated to Flp-deleter mice to remove the neomycin selection cassette. |