| First Author | Tucker KL | Year | 1997 |
| Journal | Nucleic Acids Res | Volume | 25 |
| Issue | 18 | Pages | 3745-6 |
| PubMed ID | 9278500 | Mgi Jnum | J:92995 |
| Mgi Id | MGI:3055467 | Doi | 10.1093/nar/25.18.3745 |
| Citation | Tucker KL, et al. (1997) A transgenic mouse strain expressing four drug-selectable marker genes. Nucleic Acids Res 25(18):3745-6 |
| abstractText | Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells. |
