| Primary Identifier | MGI:1857304 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Apob |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Levels of LDL-cholesterol, VLDL, and triglyceride LDL are higher relative to wild-type mice in APOB100 only mice in the presence of wild-type APOE production. In the presence of genetic APOE deficiency, APOB100 only mice have lowered cholesterol levels (J:33830). Phenotypic Similarity to Human Syndrome: Calcific Aortic Valve Disease J:227165 in mice homozygous for Apobtm2Sgy and Ldlrtm1Her and carrying the Tg(Ins-Igf2)1Fbos transgene. |
| molecularNote | A "hit and run"-type vector was used to create a CTA to TTA mutation in codon 2179 (codon 2153 in the original publication) in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB100 isoform is unaffected by this mutation, while no ApoB48 isoform is produced from this allele. |
