| First Author | West EE | Year | 2023 |
| Journal | Immunity | Volume | 56 |
| Issue | 9 | Pages | 2036-2053.e12 |
| PubMed ID | 37572656 | Mgi Jnum | J:340613 |
| Mgi Id | MGI:7528593 | Doi | 10.1016/j.immuni.2023.07.014 |
| Citation | West EE, et al. (2023) Loss of CD4(+) T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection. Immunity 56(9):2036-2053.e12 |
| abstractText | Arginase 1 (Arg1), the enzyme catalyzing the conversion of arginine to ornithine, is a hallmark of IL-10-producing immunoregulatory M2 macrophages. However, its expression in T cells is disputed. Here, we demonstrate that induction of Arg1 expression is a key feature of lung CD4(+) T cells during mouse in vivo influenza infection. Conditional ablation of Arg1 in CD4(+) T cells accelerated both virus-specific T helper 1 (Th1) effector responses and its resolution, resulting in efficient viral clearance and reduced lung pathology. Using unbiased transcriptomics and metabolomics, we found that Arg1-deficiency was distinct from Arg2-deficiency and caused altered glutamine metabolism. Rebalancing this perturbed glutamine flux normalized the cellular Th1 response. CD4(+) T cells from rare ARG1-deficient patients or CRISPR-Cas9-mediated ARG1-deletion in healthy donor cells phenocopied the murine cellular phenotype. Collectively, CD4(+) T cell-intrinsic Arg1 functions as an unexpected rheostat regulating the kinetics of the mammalian Th1 lifecycle with implications for Th1-associated tissue pathologies. |
