| First Author | Chatterjee G | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 372 |
| Issue | 3 | Pages | 412-7 |
| PubMed ID | 18498762 | Mgi Jnum | J:137884 |
| Mgi Id | MGI:3803091 | Doi | 10.1016/j.bbrc.2008.05.053 |
| Citation | Chatterjee G, et al. (2008) Epithelial V-like antigen regulates permeability of the blood-CSF barrier. Biochem Biophys Res Commun 372(3):412-7 |
| abstractText | Epithelial V-like antigen (EVA), a CD3-binding immunoglobulin-like protein, regulates embryonic thymic development. Here we demonstrate that EVA is expressed in choroid plexus from mature immune competent and lymphocyte-deficient (RAG-/-) mice. Choroid plexus epithelial cells from RAG-/- mice demonstrated reduced junctional integrity and enhanced permeability that was associated with decreased expression of E-cadherin and EVA mRNA as compared to wild-type mice. Following iv infusion of an anti-CD3 antibody (145-2C11) that also binds EVA, expression of E-cadherin and EVA mRNA approached levels seen in wild-type mice. Immuno-fluorescent staining for cadherin also revealed decreased expression in untreated RAG-/- mice that could be increased by 145-2C11 treatment. Expression of mouse EVA in HEK-293 cells followed by challenge with 145-2C11 resulted in increased cytosolic calcium that was not seen in control cells. These results suggest that EVA expressed in choroid plexus cells may regulate the permeability of the blood-CSF barrier. |
