| First Author | Borghesi L | Year | 2004 |
| Journal | J Exp Med | Volume | 199 |
| Issue | 4 | Pages | 491-502 |
| PubMed ID | 14769852 | Mgi Jnum | J:119401 |
| Mgi Id | MGI:3702056 | Doi | 10.1084/jem.20031800 |
| Citation | Borghesi L, et al. (2004) B lineage-specific regulation of V(D)J recombinase activity is established in common lymphoid progenitors. J Exp Med 199(4):491-502 |
| abstractText | Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during hematopoietic development the V(D)J recombinase is first active, nor is it known what elements activate the recombinase in multipotent hematopoietic progenitors. Using mice that express a fluorescent transgenic V(D)J recombination reporter, we show that the V(D)J recombinase is active as early as common lymphoid progenitors (CLPs) but not in the upstream progenitors that retain myeloid lineage potential. Evidence of this recombinase activity is detectable in all four progeny lineages (B, T, and NK, and DC), and rag2 levels are the highest in progenitor subsets immediately downstream of the CLP. By single cell PCR, we demonstrate that V(D)J rearrangements are detectable at IgH loci in approximately 5% of splenic natural killer cells. Finally, we show that recombinase activity in CLPs is largely controlled by the Erag enhancer. As activity of the Erag enhancer is restricted to the B cell lineage, this provides the first molecular evidence for establishment of a lineage-specific transcription program in multipotent progenitors. |
