| First Author | Manjunath N | Year | 1995 |
| Journal | Nature | Volume | 377 |
| Issue | 6549 | Pages | 535-8 |
| PubMed ID | 7566153 | Mgi Jnum | J:29272 |
| Mgi Id | MGI:76803 | Doi | 10.1038/377535a0 |
| Citation | Manjunath N, et al. (1995) Negative regulation of T-cell adhesion and activation by CD43. Nature 377(6549):535-8 |
| abstractText | CD43 is a cell-surface sialoglycoprotein expressed by a variety of haematopoietically derived cells, including T lymphocytes. Earlier observations of defective CD43 expression by T lymphocytes from boys with the X-chromosome-linked Wiskott-Aldrich syndrome suggested the importance of CD43 in lymphocyte function. Subsequent studies have suggested that CD43 facilitates leukocyte adhesion and has a co-stimulatory role during T-cell activation. To define the physiologically relevant function(s) of CD43, we have generated CD43-knockout mice. We report here that CD43-deficient T cells from such mice show a marked increase in their in vitro proliferative response to concanavalin A, anti-CD3, the superantigen SEB and allostimulation. Additionally, CD43-deficient T cells show a substantial enhancement in homotypic adhesion and in their ability to bind different ligands, including fibronectin and the intercellular adhesion molecule ICAM-1. Vaccinia-virus-infected CD43-knockout mice mounted an augmented anti-vaccinia cytotoxic T-cell response compared with their wild-type littermates, yet developed an increased virus load. We conclude that CD43 negatively regulates T-cell activation and adhesion and is important for viral clearance. |
