| First Author | Imig C | Year | 2014 |
| Journal | Neuron | Volume | 84 |
| Issue | 2 | Pages | 416-31 |
| PubMed ID | 25374362 | Mgi Jnum | J:218225 |
| Mgi Id | MGI:5617001 | Doi | 10.1016/j.neuron.2014.10.009 |
| Citation | Imig C, et al. (2014) The morphological and molecular nature of synaptic vesicle priming at presynaptic active zones. Neuron 84(2):416-31 |
| abstractText | Synaptic vesicle docking, priming, and fusion at active zones are orchestrated by a complex molecular machinery. We employed hippocampal organotypic slice cultures from mice lacking key presynaptic proteins, cryofixation, and three-dimensional electron tomography to study the mechanism of synaptic vesicle docking in the same experimental setting, with high precision, and in a near-native state. We dissected previously indistinguishable, sequential steps in synaptic vesicle active zone recruitment (tethering) and membrane attachment (docking) and found that vesicle docking requires Munc13/CAPS family priming proteins and all three neuronal SNAREs, but not Synaptotagmin-1 or Complexins. Our data indicate that membrane-attached vesicles comprise the readily releasable pool of fusion-competent vesicles and that synaptic vesicle docking, priming, and trans-SNARE complex assembly are the respective morphological, functional, and molecular manifestations of the same process, which operates downstream of vesicle tethering by active zone components. |
