| First Author | Seaman MS | Year | 2000 |
| Journal | J Immunol | Volume | 165 |
| Issue | 9 | Pages | 5192-201 |
| PubMed ID | 11046052 | Mgi Jnum | J:118922 |
| Mgi Id | MGI:3700641 | Doi | 10.4049/jimmunol.165.9.5192 |
| Citation | Seaman MS, et al. (2000) MHC class Ib-restricted CTL provide protection against primary and secondary Listeria monocytogenes infection. J Immunol 165(9):5192-201 |
| abstractText | Infection of B6 mice with the intracellular pathogen Listeria monocytogenes (LM) results in the activation of CD8(+) T cells that respond to Ag presented by both MHC class Ia and class Ib molecules. Enzyme-linked immunospot analysis reveals that these CTL populations expand and contract at different times following a primary sublethal LM infection. Between days 4 and 6 postinfection, class Ib-restricted CTL exhibit a rapid proliferative response that is primarily H2-M3 restricted. The peak response of class Ia-restricted CD8(+) T cells occurs a few days later, after the majority of bacteria have been cleared. Although class Ia-restricted CTL exhibit a vigorous recall response to secondary LM infection, we observe limited expansion of class Ib-restricted memory CTL, even in MHC class Ia-deficient mice (B6.K(b-/-)D(b-/-)). Despite this lack of enhanced expansion in vivo, class Ib-restricted memory CTL retain the ability to proliferate and expand when provided with Ag in vitro. Furthermore, we demonstrate that in vivo depletion of CD8(+) T cells in LM-immune B6.K(b-/-)D(b-/-) mice severely impairs memory protection. Together, these data demonstrate that class Ib-restricted CTL play an important role in clearing a primary LM infection and generate a memory population capable of providing significant protection against subsequent infection. |
