| First Author | Paliard X | Year | 2001 |
| Journal | Virology | Volume | 282 |
| Issue | 1 | Pages | 56-64 |
| PubMed ID | 11259190 | Mgi Jnum | J:68353 |
| Mgi Id | MGI:1932598 | Doi | 10.1006/viro.2000.0829 |
| Citation | Paliard X, et al. (2001) Induction of herpes simplex virus gB-specific cytotoxic T lymphocytes in TAP1-deficient mice by genetic immunization but not HSV infection. Virology 282(1):56-64 |
| abstractText | Loading of most endogenous peptides on major histocompatibility complex class I molecules is conditional on their transport into the endoplasmic reticulum (ER) by the peptide transporter TAP. We describe an HSV-2/1 cross-reactive cytotoxic T-cell (CTL) epitope that is processed in a TAP1-independent manner in vivo following immunization of TAP1-/- mice with naked DNA or a recombinant vaccinia virus. These data indicated that TAP1-independent processing of endogenous proteins is sufficient to prime CTLs in vivo. TAP1-independent processing of this epitope was not due to ER targeting by signal sequences and exogenous loading of MHC-I molecules and was not influenced by the amino acids flanking this epitope. In contrast, TAP1-/- mice infected with HSV-2 or HSV-2 mutants did not mount a CTL response against this epitope. Copyright 2001 Academic Press. |
