| First Author | Fedorov LM | Year | 2006 |
| Journal | Mech Ageing Dev | Volume | 127 |
| Issue | 7 | Pages | 600-9 |
| PubMed ID | 16620920 | Mgi Jnum | J:110243 |
| Mgi Id | MGI:3639666 | Doi | 10.1016/j.mad.2006.02.009 |
| Citation | Fedorov LM, et al. (2006) Renal failure causes early death of bcl-2 deficient mice. Mech Ageing Dev 127(7):600-9 |
| abstractText | BCL-2 functions as a death repressor molecule in an evolutionary conserved cell death pathway. Inactivation of bcl-2 in mice results in pleiotropic effects including postnatal growth retardation, massive apoptosis in lymphoid tissues, polycystic kidney disease (PKD) and shortened lifespan. To evaluate the influence of the affected bcl-2 deficient kidneys on the postnatal development and lifespan of bcl-2 knockout mice we used 'the rescue of (n-1) affected tissues' strategy. According to this strategy bcl-2 heterozygous animals were crossed with H2K-hbcl-2 transgenic mice expressing human BCL-2 in most tissues and organs excluding the kidney. Overexpression of hBCL-2 in bcl-2-/- mice rescues growth retardation, normalizes and protects the hematolymphoid system from gamma-radiation. However, the hbcl-2 transgene is not expressed in kidneys and the rescued mice have PKD and a shortened lifespan. Thus, our results indicated that PKD is the main reason of early mortality in bcl-2 deficient mice. Moreover, we have created mouse model, similar to the kidney specific knockout of bcl-2. Such models can be useful to study the influence of bcl-2 or other gene deficiency in individual organs (or tissues) on development and ageing of whole organism. |
