| First Author | Kaye J | Year | 2004 |
| Journal | Transplantation | Volume | 77 |
| Issue | 2 | Pages | 314-6 |
| PubMed ID | 14743001 | Mgi Jnum | J:88310 |
| Mgi Id | MGI:3032793 | Doi | 10.1097/01.TP.0000101008.13282.FD |
| Citation | Kaye J, et al. (2004) The role of complement receptors in production of antibodies specific for Galalpha1,3Gal. Transplantation 77(2):314-6 |
| abstractText | By using alpha-galactosyl transferase knockout (GT-/-) mice, which make natural alphaGal-reactive antibodies, we examined the role of complement receptors in the production of alphaGal-specific antibodies. GT-/- mice were crossed with complement receptor 2 loci knockout mice to generate double knockout (DKO) mice. alphaGal-specific natural antibodies were detectable by enzyme-linked immunosorbent assay in the serum of GT-/- mice by 9 weeks of age. In contrast, only low titers of alphaGal-specific natural antibodies were detectable only in the serum of older DKO mice. Serum titers of alphaGal-reactive antibodies in GT-/- mice increased significantly after immunization with pig cells. In contrast, immunization had little effect on alphaGal-reactive antibody levels in DKO mice. Similarly, pretreatment of GT-/- mice with a blocking antibody to CD21 and CD35 inhibited production of alphaGal-reactive antibodies after immunization. However, DKO mice were able to make alphaGal-specific antibodies after secondary immunization. Thus, Cr2 loci-encoded receptors seem to be directly involved in the production of primary alphaGal-reactive antibodies. |
