| First Author | Mazzon E | Year | 2008 |
| Journal | Am J Physiol Gastrointest Liver Physiol | Volume | 294 |
| Issue | 5 | Pages | G1268-80 |
| PubMed ID | 18308862 | Mgi Jnum | J:136584 |
| Mgi Id | MGI:3796669 | Doi | 10.1152/ajpgi.00014.2008 |
| Citation | Mazzon E, et al. (2008) Role of TNF-alpha in ileum tight junction alteration in mouse model of restraint stress. Am J Physiol Gastrointest Liver Physiol 294(5):G1268-80 |
| abstractText | Restraint stress induces permeability changes in the small intestine, but little is known about the role of tumor necrosis factor (TNF)-alpha in the defects of the TJ function. In the present study, we used tumor necrosis factor-R1 knockout mice (TNF-alpha-R1KO) to understand the roles of TNF-alpha on ileum altered permeability function in models of immobilization stress. The genetic TNF-alpha inhibition significantly reduced the degree of 1) TNF-alpha production in ileum tissues; 2) the alteration of zonula occludens-1 (ZO-1), claudin-2, claudin-4, claudin-5, and beta-catenin (immunohistochemistry); and 3) apoptosis (TUNEL staining, Bax, Bcl-2 expression). Taken together, our results demonstrate that inhibition of TNF-alpha reduces the tight junction permeability in the ileum tissues associated with immobilization stress, suggesting a possible role of TNF-alpha on ileum barrier dysfunction. |
