| First Author | Kinjo R | Year | 2022 |
| Journal | Int J Mol Sci | Volume | 23 |
| Issue | 6 | PubMed ID | 35328385 |
| Mgi Jnum | J:322861 | Mgi Id | MGI:7259155 |
| Doi | 10.3390/ijms23062968 | Citation | Kinjo R, et al. (2022) Micro-Osteoperforations Induce TNF-alpha Expression and Accelerate Orthodontic Tooth Movement via TNF-alpha-Responsive Stromal Cells. Int J Mol Sci 23(6) |
| abstractText | Micro-osteoperforations (MOPs) have been reported to accelerate orthodontic tooth movement (OTM), and tumor necrosis factor (TNF)-alpha has been reported to play a crucial role in OTM. In this report, the influence of MOPs during OTM was analyzed. We evaluated the expression of TNF-alpha with and without MOPs by RT-PCR analysis. A Ni-Ti closed coil spring was fixed between the maxillary left first molar and the incisors as an OTM mouse model to move the first molar in the mesial direction. MOPs were prepared on the lingual side and mesial side of the upper first molars. Furthermore, to investigate the target cell of TNF-alpha for osteoclast formation during OTM with MOPs in vivo, we created four types of chimeric mice in which bone marrow of wild-type (WT) or TNF receptor 1- and 2-deficient mice (KO) was transplanted into lethally irradiated WT or KO mice. The results showed that MOPs increased TNF-alpha expression, the distance of tooth movement and osteoclast formation significantly. Furthermore, mice with TNF-alpha-responsive stromal cells showed a significant increase in tooth movement and number of osteoclasts by MOPs. We conclude that MOPs increase TNF-alpha expression, and tooth movement is dependent on TNF-alpha-responsive stromal cells. |
