| First Author | Shabalina IG | Year | 2013 |
| Journal | Cell Rep | Volume | 5 |
| Issue | 5 | Pages | 1196-203 |
| PubMed ID | 24290753 | Mgi Jnum | J:206840 |
| Mgi Id | MGI:5553032 | Doi | 10.1016/j.celrep.2013.10.044 |
| Citation | Shabalina IG, et al. (2013) UCP1 in brite/beige adipose tissue mitochondria is functionally thermogenic. Cell Rep 5(5):1196-203 |
| abstractText | The phenomenon of white fat "browning," in which certain white adipose tissue depots significantly increase gene expression for the uncoupling protein UCP1 and thus supposedly acquire thermogenic, fat-burning properties, has attracted considerable attention. Because the mRNA increases are from very low initial levels, the metabolic relevance of the change is unclear: is the UCP1 protein thermogenically competent in these brite/beige-fat mitochondria? We found that, in mitochondria isolated from the inguinal "white" adipose depot of cold-acclimated mice, UCP1 protein levels almost reached those in brown-fat mitochondria. The UCP1 was thermogenically functional, in that these mitochondria exhibited UCP1-dependent thermogenesis with lipid or carbohydrate substrates with canonical guanosine diphosphate (GDP) sensitivity and loss of thermogenesis in UCP1 knockout (KO) mice. Obesogenic mouse strains had a lower thermogenic potential than obesity-resistant strains. The thermogenic density (UCP1-dependent oxygen consumption per g tissue) of inguinal white adipose tissue was maximally one-fifth of interscapular brown adipose tissue, and the total quantitative contribution of all inguinal mitochondria was maximally one-third of all interscapular brown-fat mitochondria, indicating that the classical brown adipose tissue depots would still predominate in thermogenesis. |
