| First Author | Goicoechea L | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 1 | Pages | 101068 |
| PubMed ID | 35024626 | Mgi Jnum | J:353179 |
| Mgi Id | MGI:7664284 | Doi | 10.1016/j.xpro.2021.101068 |
| Citation | Goicoechea L, et al. (2022) GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver. STAR Protoc 3(1):101068 |
| abstractText | Abnormal cholesterol metabolism is linked to many neurodegenerative disorders. Here, we present a protocol for the production of a recombinant protein consisting of a Glutathione-S-Transferase tag fused with the Perfringolysin O (PFO). The GST-PFO tag enables analysis of the localization of cholesterol in subcellular membranes of human and mice brain and liver tissues. We have used this approach for samples from Niemann-Pick type C disease and non-alcoholic steatohepatitis models. The construct may also have applications for the diagnosis of cholesterol-accumulating disorders. For complete details on the use and execution of this protocol, please refer to Kwiatkowska et al. (2014). |
