| Primary Identifier | MGI:1857618 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Nkx2-5 |
| Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
| Is Recombinase | false | Is Wild Type | false |
| description | Homozygous mutant ES cells were injected into C57BL/6 blastocysts to form chimeric animals. Mutant ES cells were shown to contribute to most tissues of surviving animals except heart. Embryos with more than 30-40% contribution of mutant cells to the heart exhibit the phenotype of the homozygous mutant. Embryos with a 20-30% contribution of mutant ES cells to the heart exhibited milder phenotypic defects than the homozygous mutant. Embryos with less than 15% contribution of mutant cells to the heart exhibit no significant heart defects at E10.5(J:52597). |
| molecularNote | A lacZ-PGK-neomycin resistance cassette replaced all of the coding sequences. |
