| First Author | Guillot PV | Year | 1997 |
| Journal | Genet Res | Volume | 70 |
| Pages | 84 (Abstr) | Mgi Jnum | J:44121 |
| Mgi Id | MGI:1099372 | Citation | Guillot PV, et al. (1997) Genetic mapping of the doublefoot (Dbf) mutation. Genet Res 70:84 (Abstr) |
| abstractText | Full text of Abstract: Genetic mapping of the doublefoot (Dbf) mutation. PASCALE-VALERIE GUILLOT, R. QUINNEY, P.H. GLENISTER, S. KERSCHER, Y. BOYD and M.F. LYON. Mammalian Genetics Unit, MRC, Harwell, Didcot, Oxfordshire OX11 ORD, UK. An F1 male offspring carrying a spontaneous mutation was found in a C3H/HeH x 101/H cross mating. The mutation is characterized by extra toes on all four feet, hence the name doublefoot, Dbf. Previous work has shown that the mutant allele is dominant and maps at a new locus on chromosome 1, between fuzzy (fz) and leaden (ln). Linkage tests with microsatellite markers located between fz and ln (D1Mit22, D1Mit77, D1Mit24, D1Mit8) carried out on offspring from an interspecific backcross with Mus spretus showed that Dbf locus maps 7.3 +/- 1.8 cM away from D1Mit22 and 5.9 +/- 1.6 cM away from D1Mit8 and close to D1Mit24 and D1Mit77. It does not recombine with either of these markers, which in the Whitehead map are about 4 cM apart. However, this difference is not an effect of Dbf, as shown by results obtained in a control backcross. Among the genes mapped around the Dbf locus, Pax3 might be a candidate gene. However, the analysis of the segregation pattern of eight mice, known to be recombinant between D1Mit22 and D1Mit8 showed three recombinants between Pax3 and Dbf, leading to Pax3 being ruled out as a candidate gene for the Dbf phenotype. |
