| First Author | Lyon MF | Year | 1983 |
| Journal | Mouse News Lett | Volume | 68 |
| Pages | 68 | Mgi Jnum | J:13933 |
| Mgi Id | MGI:62111 | Citation | Lyon MF, et al. (1983) Dominant haemolytic anemia. Mouse News Lett 68:68 |
| abstractText | Full text of MNL contribution: 3. Dominant haemolytic anaemia. In an experiment in which male mice were treated with 250 mg/kg ethylnitrosourea, and offspring were obtained from sperm treated at the stem cell stage, one offspring was found with marked anaemia at birth. The anaemia persisted throughout life and was shown to be due to an autosomal dominant gene, given the provisional name and symbol neonatal anaemia, Nan. Heterozygotes show reasonably normal viability and fertility. Homozygotes die at 10-11 days gestation with severe lack of haemopoiesis. The anaemia in the heterozygotes is of the haemolytic type. The RBC count is moderately reduced, with anisocytosis, and many nucleated cells. The spleen is enlarged with hyperactive haemopoiesis. Iron was present in liver and spleen and there were marked iron deposits in the proximal tubule of the kidney. When radiation chimaeras were made by injecting spleen cells from Nan/+ into heavily irradiated normal hosts the chimaeras showed an anaemia typical of the Nan/+ donor. In addition, donor cell enzyme variants and haemoglobin type were present in blood and lymphomyeloid tissues. Thus the anaemia is of the "intrinsic" type. Measurements of red cell osmotic fragility showed that Nan/+ cells were more fragile than +/+. The NaCl concentration at which 50% of Nan/+ red cells lysed was 0.62 +/- 0.02% (n = 5) whereas the 50% point for +/+ was 0.54 +/- 0.02% (n = 5). The gene is not allelic with Hba or Hbb and not linked with T, Xt, W, a, cch or d se. Further studies are in progress. (Lyon, Glenister, Loutit and Peters) |
