| First Author | Hegyi Z | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 10562 |
| PubMed ID | 30002493 | Mgi Jnum | J:313201 |
| Mgi Id | MGI:6791614 | Doi | 10.1038/s41598-018-28763-6 |
| Citation | Hegyi Z, et al. (2018) CB1 receptor activation induces intracellular Ca(2+) mobilization and 2-arachidonoylglycerol release in rodent spinal cord astrocytes. Sci Rep 8(1):10562 |
| abstractText | Accumulating evidence supports the role of astrocytes in endocannabinoid mediated modulation of neural activity. It has been reported that some astrocytes express the cannabinoid type 1 receptor (CB1-R), the activation of which is leading to Ca(2+) mobilization from internal stores and a consecutive release of glutamate. It has also been documented that astrocytes have the potential to produce the endocannabinoid 2-arachidonoylglycerol, one of the best known CB1-R agonist. However, no relationship between CB1-R activation and 2-arachidonoylglycerol production has ever been demonstrated. Here we show that rat spinal astrocytes co-express CB1-Rs and the 2-arachidonoylglycerol synthesizing enzyme, diacylglycerol lipase-alpha in close vicinity to each other. We also demonstrate that activation of CB1-Rs induces a substantial elevation of intracellular Ca(2+) concentration in astrocytes. Finally, we provide evidence that the evoked Ca(2+) transients lead to the production of 2-arachidonoylglycerol in cultured astrocytes. The results provide evidence for a novel cannabinoid induced endocannabinoid release mechanism in astrocytes which broadens the bidirectional signaling repertoire between astrocytes and neurons. |
