| First Author | Chen Y | Year | 2022 |
| Journal | J Appl Oral Sci | Volume | 30 |
| Pages | e20220076 | PubMed ID | 35830121 |
| Mgi Jnum | J:334020 | Mgi Id | MGI:7443249 |
| Doi | 10.1590/1678-7757-2022-0076 | Citation | Chen Y, et al. (2022) In vitro and in vivo study of the pathogenic role of PPARalpha in experimental periodontitis. J Appl Oral Sci 30:e20220076 |
| abstractText | OBJECTIVE: The purpose of this study is to investigate the pathogenic role of PPARalpha in periodontal antigen treated gingival cells in vitro and in experimental periodontitis in vivo . METHODOLOGY: Gingival fibroblasts, gingival epithelial cells and splenocytes were isolated from C57BL/6J wild type (WT) mice and treated with fixed P. gingivalis at for 48 hours. The mRNA levels of PPARs, TNFalpha, IL-1beta and IL-10 were detected by Real-time quantitative PCR. Silk ligatures after being soaked in the P.gingivalis suspension were tied around both maxillary second molars of WT mice or PPARalpha knock-out (KO) mice for two weeks. PPARalpha agonist fenofibrate and vehicle control were injected into the different side of the palatal gingiva on days 3, 6, and 9. At day 14, bone resorption and gingival mRNA expression levels of PPARs, TNFalpha, IL-1beta and IL-10 were measured by micro-computed tomography and RT-qPCR respectively. RESULTS: P. gingivalis treatment downregulated the expression of PPARalpha, but not PPARbeta or PPARgamma, and increased the expression of TNF-alpha and IL-1beta in Gingival fibroblasts, gingival epithelial cells and splenocytes from WT mice. Gingival mRNA levels of PPARalpha were significantly decreased in experimental periodontitis in WT mice. The bone loss of PPARalpha KO mice in experimental periodontitis was significantly higher than WT mice and was not reduced by fenofibrate treatment. Gingival TNFalpha protein expressions were significantly increased by P. gingivalis associated ligation and decreased by fenofibrate treatment in WT mice but not in PPARalpha KO mice. CONCLUSION: This study suggests that PPARalpha plays an essential role in periodontitis. |
