| First Author | Monory K | Year | 2002 |
| Journal | Biochem Biophys Res Commun | Volume | 292 |
| Issue | 1 | Pages | 231-5 |
| PubMed ID | 11890697 | Mgi Jnum | J:75688 |
| Mgi Id | MGI:2177370 | Doi | 10.1006/bbrc.2002.6635 |
| Citation | Monory K, et al. (2002) Novel, not adenylyl cyclase-coupled cannabinoid binding site in cerebellum of mice. Biochem Biophys Res Commun 292(1):231-5 |
| abstractText | In this study we report data suggesting the presence of a non-CB1, non-CB2 cannabinoid site in the cerebellum of CB1(minus sign/minus sign) mice. We have carried out [(35)S]GTPgammaS binding experiments in striata, hippocampi, and cerebella of CB1(minus sign/minus sign) and CB1(+/+) mice with Delta(9)-THC, WIN55,212-2, HU-210, SR141716A, and SR144528. In CB1(minus sign/minus sign) mice Delta(9)-THC and HU-210 did not stimulate [(35)S]GTPgammaS binding. However, WIN55,212-2 was able to stimulate [(35)S]GTPgammaS binding in cerebella of CB1(minus sign/minus sign) mice. The maximal effect of this stimulation was 31% that of wild type animals. This effect was reversible neither by CB1 nor CB2 receptor antagonists. Similar results were obtained with the endogenous cannabinoid, anandamide. However, adenylyl cyclase was not inhibited by WIN55,212-2 or anandamide in the CB1(minus sign/minus sign) animals. In striata and hippocampi of CB1(minus sign/minus sign) mice no [(35)S]GTPgammaS stimulation curve could be obtained with WIN55,212. Our findings suggest that there is a non-CB1 non-CB2 receptor present in the cerebellum of CB1(minus sign/minus sign) mice. (C)2002 Elsevier Science (USA). |
