| First Author | Dragoi AM | Year | 2005 |
| Journal | EMBO J | Volume | 24 |
| Issue | 4 | Pages | 779-89 |
| PubMed ID | 15678105 | Mgi Jnum | J:96474 |
| Mgi Id | MGI:3530697 | Doi | 10.1038/sj.emboj.7600539 |
| Citation | Dragoi AM, et al. (2005) DNA-PKcs, but not TLR9, is required for activation of Akt by CpG-DNA. EMBO J 24(4):779-89 |
| abstractText | CpG-DNA and its related synthetic CpG oligodeoxynucleotides (CpG-ODNs) play an important role in immune cell survival. It has been suggested that Akt is one of the CpG-DNA-responsive serine/threonine kinases; however, the target protein of CpG-DNA that leads to Akt activation has not been elucidated. Here, we report that ex vivo stimulation of bone marrow-derived macrophages (BMDMs) from mice lacking the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) results in defective phosphorylation and activation of Akt by CpG-DNA. Unexpectedly, loss of the Toll-like receptor 9 has a minimal effect on Akt activation in response to CpG-DNA. Further in vitro analysis using purified DNA-PK and recombinant Akt proteins reveals that DNA-PK directly induces phosphorylation and activation of Akt. In addition, in BMDMs, DNA-PKcs associates with Akt upon CpG-DNA stimulation and triggers transient nuclear translocation of Akt. Thus, our findings establish a novel role for DNA-PKcs in CpG-DNA signaling and define a CpG-DNA/DNA-PKcs/Akt pathway. |
