| First Author | Nakanishi Y | Year | 2018 |
| Journal | Immunity | Volume | 49 |
| Issue | 6 | Pages | 1132-1147.e7 |
| PubMed ID | 30552022 | Mgi Jnum | J:305332 |
| Mgi Id | MGI:6706708 | Doi | 10.1016/j.immuni.2018.09.013 |
| Citation | Nakanishi Y, et al. (2018) Simultaneous Loss of Both Atypical Protein Kinase C Genes in the Intestinal Epithelium Drives Serrated Intestinal Cancer by Impairing Immunosurveillance. Immunity 49(6):1132-1147.e7 |
| abstractText | Serrated adenocarcinoma, an alternative pathway for colorectal cancer (CRC) development, accounts for 15%-30% of all CRCs and is aggressive and treatment resistant. We show that the expression of atypical protein kinase C zeta (PKCzeta) and PKClambda/iota was reduced in human serrated tumors. Simultaneous inactivation of the encoding genes in the mouse intestinal epithelium resulted in spontaneous serrated tumorigenesis that progressed to advanced cancer with a strongly reactive and immunosuppressive stroma. Whereas epithelial PKClambda/iota deficiency led to immunogenic cell death and the infiltration of CD8(+) T cells, which repressed tumor initiation, PKCzeta loss impaired interferon and CD8(+) T cell responses, which resulted in tumorigenesis. Combined treatment with a TGF-beta receptor inhibitor plus anti-PD-L1 checkpoint blockade showed synergistic curative activity. Analysis of human samples supported the relevance of these kinases in the immunosurveillance defects of human serrated CRC. These findings provide insight into avenues for the detection and treatment of this poor-prognosis subtype of CRC. |
