| First Author | Meyers EN | Year | 1998 |
| Journal | Nat Genet | Volume | 18 |
| Issue | 2 | Pages | 136-41 |
| PubMed ID | 9462741 | Mgi Jnum | J:45909 |
| Mgi Id | MGI:1196692 | Doi | 10.1038/ng0298-136 |
| Citation | Meyers EN, et al. (1998) An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat Genet 18(2):136-41 |
| abstractText | We describe a strategy for generating an allelic series of mutations at a given locus that requires the production of only one targetted mouse line. The 'allelogenic' mouse line we produced carries a hypomorphic allele of Fgf8, which can be converted to a null allele by mating to cre transgenic animals. The hypomorphic allele can also be reverted to wild-type by mating the allelogenic mice to flp transgenic animals, thereby generating a mouse line suitable for Cre-induced tissue-specific knockout experiments. Analysis of embryos carrying different combinations of these alleles revealed requirements for Fgf8 gene function during gastrulation, as well as cardiac, craniofacial, forebrain, midbrain and cerebellar development. |
