| First Author | Hérault Y | Year | 1998 |
| Journal | Nat Genet | Volume | 20 |
| Issue | 4 | Pages | 381-4 |
| PubMed ID | 9843213 | Mgi Jnum | J:51289 |
| Mgi Id | MGI:1314991 | Doi | 10.1038/3861 |
| Citation | Herault Y, et al. (1998) Engineering chromosomes in mice through targeted meiotic recombination (TAMERE). Nat Genet 20(4):381-4 |
| abstractText | Functional studies of large transcription units, clustered genes and chromosomal loci require the design of novel experimental toots to engineer genomic macro- rearrangements. Here, we present a strategy to produce deficiencies or duplications by crossing mice carrying loxP sites in homologous loci. This trans-allelic targeted meiotic recombination (TAMERE) protocol allows for the combination of various alleles within a particular locus as well as for generation of interchromosomal unequal exchanges. Novel genetic configurations can thus be produced without multiple targeting and selection steps in embryonic stem (ES) cells. A concomitant deletion/duplication event of the Hoxd12 locus shows the potential of this approach. The high frequency of such targeted exchanges in vivo makes TAMERE a powerful genetic tool applicable to research areas in which complex genomic modifications are required. |
