| First Author | Yélamos J | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 324 |
| Issue | 2 | Pages | 840-8 |
| PubMed ID | 15474504 | Mgi Jnum | J:113767 |
| Mgi Id | MGI:3687626 | Doi | 10.1016/j.bbrc.2004.09.135 |
| Citation | Yelamos J, et al. (2004) Genetic and pharmacological inhibition of poly(ADP-ribose) polymerase-1 interferes in the chlamydial life cycle. Biochem Biophys Res Commun 324(2):840-8 |
| abstractText | Chlamydiaceae are intracellular bacteria responsible for a variety of infections, ranging from asymptomatic to very severe, in humans and animals. We have investigated the role of poly(ADP-ribose) polymerase-1 (PARP-1) in Chlamydophila abortus infection using PARP-1-/- and their littermates PARP-1+/+ mice. Infection was resolved more efficiently by PARP-1-/- than PARP-1+/+ mice. However, the inflammatory response was similar in both strains, suggesting a potential role for PARP-1 in the cross-talk between this microorganism and the host cells. PARP-1-/- fibroblasts showed a 10-fold lower rate of chlamydiae production than PARP-1+/+. Moreover, a strong inhibition of bacterial production was also observed after pharmacological inhibition of PARP-1 activity in McCoy cells. Likewise, PARP-1 inhibition induced a higher level of cell death of infected cells, interfering in this way with the normal bacterial cell cycle. Overall, we identify PARP-1 as a new molecule involved in chlamydial developmental cycle, although the intrinsic mechanisms deserve further studies. |
