| First Author | Son DI | Year | 2020 |
| Journal | Biochem Biophys Res Commun | Volume | 526 |
| Issue | 4 | Pages | 986-992 |
| PubMed ID | 32295715 | Mgi Jnum | J:298496 |
| Mgi Id | MGI:6480184 | Doi | 10.1016/j.bbrc.2020.03.166 |
| Citation | Son DI, et al. (2020) PARP-1 regulates mouse embryonic neural stem cell proliferation by regulating PDGFRalpha expression. Biochem Biophys Res Commun 526(4):986-992 |
| abstractText | PARP-1 is a multifunctional enzyme that regulates DNA repair, chromatin remodeling, inflammation and cell survival. Our previous study revealed that PARP-1 is required for maintaining normal level of neural stem cell proliferation. In the present study, we present evidence indicating that PARP-1 regulates neural stem cell proliferation by upregulating the expression of platelet-derived growth factor receptor alpha (PDGFRalpha). PARP-1 knockout neural stem cells exhibited striking downregulation of PDGFRalpha expression. We found that PARP-1 promotes the transcription of PDGFRalpha independently of its enzymatic activity. Overexpression of PDGFRalpha in the PARP-1 knockout neural stem cells reversed the proliferation defect of the knockout cells. Conversely, knockdown or blocking antibody of PDGFRalpha suppressed the proliferation of neural stem cells. In addition, blockade of PDGFRalpha increased cell death rate. Consistent with the downregulation of PDGFRalpha in the absence of PARP-1, PDGF-AA promoted proliferation of wild-type neural stem cells but not that of PARP-1 knockout cells. These results suggest that PARP-1 can control the neural stem cell proliferation by regulating the expression of PDGFRalpha. |
