| First Author | Taki S | Year | 2024 |
| Journal | Front Physiol | Volume | 15 |
| Pages | 1406448 | PubMed ID | 38952869 |
| Mgi Jnum | J:350495 | Mgi Id | MGI:7663477 |
| Doi | 10.3389/fphys.2024.1406448 | Citation | Taki S, et al. (2024) Novel RPTPgamma and RPTPzeta splice variants from mixed neuron-astrocyte hippocampal cultures as well as from the hippocampi of newborn and adult mice. Front Physiol 15:1406448 |
| abstractText | Receptor protein tyrosine phosphatases gamma and zeta (RPTPgamma and RPTPzeta) are transmembrane signaling proteins with extracellular carbonic anhydrase-like domains that play vital roles in the development and functioning of the central nervous system (CNS) and are implicated in tumor suppression, neurodegeneration, and sensing of extracellular [CO(2)] and [HCO(3) (-)]. RPTPgamma expresses throughout the body, whereas RPTPzeta preferentially expresses in the CNS. Here, we investigate differential RPTPgamma-RPTPzeta expression in three sources derived from a wild-type laboratory strain of C57BL/6 mice: (a) mixed neuron-astrocyte hippocampal (HC) cultures 14 days post isolation from P0-P2 pups; (b) P0-P2 pup hippocampi; and (c) 9- to 12-week-old adult hippocampi. Regarding RPTPgamma, we detect the Ptprg variant-1 (V1) transcript, representing canonical exons 1-30. Moreover, we newly validate the hypothetical assembly [XM_006517956] (propose name, Ptprg-V3), which lacks exon 14. Both transcripts are in all three HC sources. Regarding RPTPzeta, we confirm the expression of Ptprz1-V1, detecting it in pups and adults but not in cultures, and Ptprz1-V3 through Ptprz1-V7 in all three preparations. We newly validate hypothetical assemblies Ptprz1-X1 (in cultures and pups), Ptprz1-X2 (in all three), and Ptprz1-X5 (in pups and adults) and propose to re-designate them as Ptprz1-V0, Ptprz1-V2, and Ptprz1-V8, respectively. The diversity of RPTPgamma and RPTPzeta splice variants likely corresponds to distinct signaling functions, in different cellular compartments, during development vs later life. In contrast to previous studies that report divergent RPTPgamma and RPTPzeta protein expressions in neurons and sometimes in the glia, we observe that RPTPgamma and RPTPzeta co-express in the somata and processes of almost all HC neurons but not in astrocytes, in all three HC preparations. |
