| First Author | Bezman NA | Year | 2009 |
| Journal | J Immunol | Volume | 182 |
| Issue | 9 | Pages | 5183-7 |
| PubMed ID | 19380761 | Mgi Jnum | J:147721 |
| Mgi Id | MGI:3842019 | Doi | 10.4049/jimmunol.0802176 |
| Citation | Bezman NA, et al. (2009) Cutting edge: Rescue of pre-TCR but not mature TCR signaling in mice expressing membrane-targeted SLP-76. J Immunol 182(9):5183-7 |
| abstractText | SLP-76 (Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa) organizes signaling from immunoreceptors, including the platelet collagen receptor, the pre-TCR, and the TCR, and is required for T cell development. In this study we examine a mouse in which wild-type SLP-76 is replaced with a mutant constitutively targeted to the cell membrane. Membrane-targeted SLP-76 (MTS) supports ITAM signaling in platelets and from the pre-TCR. Signaling from the mature TCR, however, is defective in MTS thymocytes, resulting in failed T cell differentiation. Defective thymic selection by MTS is not rescued by a SLP-76 mutant whose localization is restricted to the cytosol. Thus, fixed localization of SLP-76 reveals differential requirements for the subcellular localization of signaling complexes downstream of the pre-TCR vs mature TCR. |
