| First Author | Wilson AJ | Year | 2014 |
| Journal | Cell Mol Life Sci | Volume | 71 |
| Issue | 1 | Pages | 165-81 |
| PubMed ID | 23708682 | Mgi Jnum | J:198665 |
| Mgi Id | MGI:5498617 | Doi | 10.1007/s00018-013-1374-5 |
| Citation | Wilson AJ, et al. (2014) Cardiomyocyte growth and sarcomerogenesis at the intercalated disc. Cell Mol Life Sci 71(1):165-81 |
| abstractText | Cardiomyocytes grow during heart maturation or disease-related cardiac remodeling. We present evidence that the intercalated disc (ID) is integral to both longitudinal and lateral growth: increases in width are accommodated by lateral extension of the plicate tread regions and increases in length by sarcomere insertion within the ID. At the margin between myofibril and the folded membrane of the ID lies a transitional junction through which the thin filaments from the last sarcomere run to the ID membrane and it has been suggested that this junction acts as a proto Z-disc for sarcomere addition. In support of this hypothesis, we have investigated the ultrastructure of the ID in mouse hearts from control and dilated cardiomyopathy (DCM) models, the MLP-null and a cardiac-specific beta-catenin mutant, cDeltaex3, as well as in human left ventricle from normal and DCM samples. We find that the ID amplitude can vary tenfold from 0.2 mum up to a maximum of ~2 mum allowing gradual expansion during heart growth. At the greatest amplitude, equivalent to a sarcomere length, A-bands and thick filaments are found within the ID membrane loops together with a Z-disc, which develops at the transitional junction position. Here, also, the tops of the membrane folds, which are rich in alphaII spectrin, become enlarged and associated with junctional sarcoplasmic reticulum. Systematically larger ID amplitudes are found in DCM samples. Other morphological differences between mouse DCM and normal hearts suggest that sarcomere inclusion is compromised in the diseased hearts. |
