| First Author | Pulliam AC | Year | 2008 |
| Journal | Exp Hematol | Volume | 36 |
| Issue | 9 | Pages | 1084-90 |
| PubMed ID | 18495331 | Mgi Jnum | J:141680 |
| Mgi Id | MGI:3819272 | Doi | 10.1016/j.exphem.2008.03.016 |
| Citation | Pulliam AC, et al. (2008) AMD3100 synergizes with G-CSF to mobilize repopulating stem cells in Fanconi anemia knockout mice. Exp Hematol 36(9):1084-90 |
| abstractText | Fanconi anemia (FA) is a heterogeneous inherited disorder characterized by a progressive bone marrow (BM) failure and susceptibility to myeloid leukemia. Genetic correction using gene-transfer technology is one potential therapy. A major hurdle in applying this technology in FA patients is the inability of granulocyte colony-stimulating factor (G-CSF) to mobilize sufficient numbers of hematopoietic stem (HSC)/progenitor cells (HPC) from the BM to the peripheral blood. Whether the low number of CD34(+) cells is a result of BM hypoplasia or an inability of G-CSF to adequately mobilize FA HSC/HPC remains incompletely understood. Here we use competitive repopulation of lethally irradiated primary and secondary recipients to show that in two murine models of FA, AMD3100 synergizes with G-CSF resulting in a mobilization of HSC, whereas G-CSF alone fails to mobilize stem cells even in the absence of hypoplasia. |
