| First Author | Kominami T | Year | 2016 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 57 |
| Issue | 3 | Pages | 1264-9 |
| PubMed ID | 26990161 | Mgi Jnum | J:257589 |
| Mgi Id | MGI:6112356 | Doi | 10.1167/iovs.15-17708 |
| Citation | Kominami T, et al. (2016) Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse. Invest Ophthalmol Vis Sci 57(3):1264-9 |
| abstractText | PURPOSE: Pikachurin is an extracellular matrix-like protein located in the synaptic cleft of photoreceptors. Pikachurin null mutant (Pika-/-) mice have abnormal ON bipolar cell function. This study aimed to determine the contribution of the ON bipolar cell pathway (ON pathway) of Pika-/- mice to the flicker ERG response and, using vector analysis, identify how the contribution varies with the stimulus frequency. METHODS: Flicker ERGs were recorded from wild-type (WT), Pika-/-, and mGluR6 null mutant (mGluR6-/-) mice. The frequency of stimulation was 3.906 to 31.250 Hz. The amplitude and phase of the fundamental components were obtained by harmonic and vector model analysis. The mGluR6-/- mice were used as a model wherein the ON pathway is known to be absent. RESULTS: The amplitudes of the fundamental components of the Pika-/- mice were significantly smaller than those of WT mice for stimulation frequencies between 3.906 and 17.578 Hz. The phase of the fundamental components of the Pika-/- mice was between those of the WT and mGluR6-/- mice. Vector analyses showed that the functioning of the ON pathway of Pika-/- mice was 12% to 25% of that of the WT mice at low frequencies (i.e., <15.625 Hz); however, it was reduced to noise level at frequencies >17.578 Hz. CONCLUSIONS: Vector model analysis can determine the degree of contribution of the ON pathway of Pika-/- mice to flicker ERG response and may be useful for determining the retinal function in mice models with abnormalities of the ON pathway. |
