| First Author | Wei W | Year | 2011 |
| Journal | Mol Cell Biol | Volume | 31 |
| Issue | 23 | Pages | 4706-19 |
| PubMed ID | 21876000 | Mgi Jnum | J:178340 |
| Mgi Id | MGI:5298163 | Doi | 10.1128/MCB.05980-11 |
| Citation | Wei W, et al. (2011) Biphasic and Dosage-Dependent Regulation of Osteoclastogenesis by beta-Catenin. Mol Cell Biol 31(23):4706-19 |
| abstractText | Wnt/beta-catenin signaling is a critical regulator of skeletal physiology. However, previous studies have mainly focused on its roles in osteoblasts, while its specific function in osteoclasts is unknown. This is a clinically important question because neutralizing antibodies against Wnt antagonists are promising new drugs for bone diseases. Here, we show that in osteoclastogenesis, beta-catenin is induced during the macrophage colony-stimulating factor (M-CSF)-mediated quiescence-to-proliferation switch but suppressed during the RANKL-mediated proliferation-to-differentiation switch. Genetically, beta-catenin deletion blocks osteoclast precursor proliferation, while beta-catenin constitutive activation sustains proliferation but prevents osteoclast differentiation, both causing osteopetrosis. In contrast, beta-catenin heterozygosity enhances osteoclast differentiation, causing osteoporosis. Biochemically, Wnt activation attenuates whereas Wnt inhibition stimulates osteoclastogenesis. Mechanistically, beta-catenin activation increases GATA2/Evi1 expression but abolishes RANKL-induced c-Jun phosphorylation. Therefore, beta-catenin exerts a pivotal biphasic and dosage-dependent regulation of osteoclastogenesis. Importantly, these findings suggest that Wnt activation is a more effective treatment for skeletal fragility than previously recognized that confers dual anabolic and anti-catabolic benefits. |
