| First Author | Dao DY | Year | 2012 |
| Journal | J Bone Miner Res | Volume | 27 |
| Issue | 8 | Pages | 1680-94 |
| PubMed ID | 22508079 | Mgi Jnum | J:320734 |
| Mgi Id | MGI:6872146 | Doi | 10.1002/jbmr.1639 |
| Citation | Dao DY, et al. (2012) Cartilage-specific beta-catenin signaling regulates chondrocyte maturation, generation of ossification centers, and perichondrial bone formation during skeletal development. J Bone Miner Res 27(8):1680-94 |
| abstractText | The WNT/beta-catenin signaling pathway is a critical regulator of chondrocyte and osteoblast differentiation during multiple phases of cartilage and bone development. Although the importance of beta-catenin signaling during the process of endochondral bone development has been previously appreciated using a variety of genetic models that manipulate beta-catenin in skeletal progenitors and osteoblasts, genetic evidence demonstrating a specific role for beta-catenin in committed growth-plate chondrocytes has been less robust. To identify the specific role of cartilage-derived beta-catenin in regulating cartilage and bone development, we studied chondrocyte-specific gain- and loss-of-function genetic mouse models using the tamoxifen-inducible Col2Cre(ERT2) transgene in combination with beta-catenin(fx(exon3)/wt) or beta-catenin(fx/fx) floxed alleles, respectively. From these genetic models and biochemical data, three significant and novel findings were uncovered. First, cartilage-specific beta-catenin signaling promotes chondrocyte maturation, possibly involving a bone morphogenic protein 2 (BMP2)-mediated mechanism. Second, cartilage-specific beta-catenin facilitates primary and secondary ossification center formation via the induction of chondrocyte hypertrophy, possibly through enhanced matrix metalloproteinase (MMP) expression at sites of cartilage degradation, and potentially by enhancing Indian hedgehog (IHH) signaling activity to recruit vascular tissues. Finally, cartilage-specific beta-catenin signaling promotes perichondrial bone formation possibly via a mechanism in which BMP2 and IHH paracrine signals synergize to accelerate perichondrial osteoblastic differentiation. The work presented here supports the concept that the cartilage-derived beta-catenin signal is a central mediator for major events during endochondral bone formation, including chondrocyte maturation, primary and secondary ossification center development, vascularization, and perichondrial bone formation. |
