| First Author | Sunny SS | Year | 2020 |
| Journal | Sci Rep | Volume | 10 |
| Issue | 1 | Pages | 9083 |
| PubMed ID | 32493941 | Mgi Jnum | J:294186 |
| Mgi Id | MGI:6452007 | Doi | 10.1038/s41598-020-65878-1 |
| Citation | Sunny SS, et al. (2020) Generation and characterization of Aldh3-Cre transgenic mice as a tool for conditional gene deletion in postnatal cornea. Sci Rep 10(1):9083 |
| abstractText | Conditional gene targeting in mice by means of Cre-loxP strategy represents a powerful approach to study mammalian gene function. This approach is however dependent on the availability of suitable strains of mice with a tissue or time restricted activity of the Cre recombinase. Here we describe Aldh3-Cre transgenic mice as a useful tool to conditionally delete genes in cornea, a specialized transparent tissue found on the anterior-most part of the eye, which acts as a protective barrier and contributes to the refractive power. Using a set of floxed alleles we demonstrate high Aldh3-Cre activity in corneal epithelial cells, corneal stroma and conjunctival epithelial cells at postnatal stages. Aldh3-Cre will thus be particularly beneficial for functional analysis of genes which are vital for postnatal development of cornea and conjunctiva. |
