| First Author | Janas ML | Year | 2011 |
| Journal | J Immunol | Volume | 187 |
| Issue | 9 | Pages | 4667-75 |
| PubMed ID | 21930962 | Mgi Jnum | J:179460 |
| Mgi Id | MGI:5302454 | Doi | 10.4049/jimmunol.1101949 |
| Citation | Janas ML, et al. (2011) Interaction of Ras with P110gamma Is required for thymic beta-selection in the mouse. J Immunol 187(9):4667-75 |
| abstractText | Thymocytes are tested for productive rearrangement of the tcrb locus by expression of a pre-TCR in a process termed beta-selection, which requires both Notch1 and CXCR4 signaling. It has been shown that activation of the GTPase Ras allows thymocytes to proliferate and differentiate in the absence of a Pre-TCR; the direct targets of Ras at this checkpoint have not been identified, however. Mice with a mutant allele of p110gamma unable to bind active Ras revealed that CXCR4-mediated PI3K activation is Ras dependent. The Ras-p110gamma interaction was necessary for efficient beta-selection-promoted proliferation but was dispensable for the survival or differentiation of thymocytes. Uncoupling Ras from p110gamma provides unambiguous identification of a Ras interaction required for thymic beta-selection. |
