| First Author | Place DE | Year | 2014 |
| Journal | PLoS One | Volume | 9 |
| Issue | 9 | Pages | e107188 |
| PubMed ID | 25198773 | Mgi Jnum | J:221522 |
| Mgi Id | MGI:5640913 | Doi | 10.1371/journal.pone.0107188 |
| Citation | Place DE, et al. (2014) Caspase-1-independent interleukin-1beta is required for clearance of Bordetella pertussis infections and whole-cell vaccine-mediated immunity. PLoS One 9(9):e107188 |
| abstractText | Whooping cough remains a significant disease worldwide and its re-emergence in highly vaccinated populations has been attributed to a combination of imperfect vaccines and evolution of the pathogen. The focus of this study was to examine the role of IL-1alpha/beta and the inflammasome in generation of the interleukin-1 (IL-1) response, which is required for the clearance of Bordetella pertussis. We show that IL-1beta but not IL-1alpha is required for mediating the clearance of B. pertussis from the lungs of mice. We further found that IL-1beta and IL-1R deficient mice, compared to wild-type, have similar but more persistent levels of inflammation, characterized by immune cell infiltration, with significantly increased IFNgamma and a normal IL-17A response during B. pertussis infection. Contrary to expectations, the cleavage of precursor IL-1beta to its mature form did not require caspase-1 during primary infections within the lung despite being required by bone marrow-derived macrophages exposed to live bacteria. We also found that the caspase-1 inflammasome was not required for protective immunity against a B. pertussis challenge following vaccination with heat-killed whole cell B. pertussis, despite IL-1R signaling being required. These findings demonstrate that caspase-1-independent host factors are involved in the processing of protective IL-1beta responses that are critical for bacterial clearance and vaccine-mediated immunity. |
