| First Author | Suzuki S | Year | 2006 |
| Journal | FEBS Lett | Volume | 580 |
| Issue | 13 | Pages | 3257-62 |
| PubMed ID | 16697375 | Mgi Jnum | J:109074 |
| Mgi Id | MGI:3625671 | Doi | 10.1016/j.febslet.2006.04.086 |
| Citation | Suzuki S, et al. (2006) Identification of TNF-alpha-responsive NF-kappaB p65-binding element in the distal promoter of the mouse serine protease inhibitor SerpinE2. FEBS Lett 580(13):3257-62 |
| abstractText | Serine protease inhibitor SerpinE2 is known as a cytokine-inducible gene. Here, we investigated whether tumor necrosis factor alpha-(TNF-alpha)-induced expression of SerpinE2 is mediated by the nuclear factor-kappaB (NF-kappaB) p65 subunit. Both steady state and TNF-alpha-induced expression of SerpinE2 mRNA were abrogated in p65(-/-) murine embryonic fibroblasts (MEFs). Reconstitution with wild-type p65 rescued SerpinE2 mRNA expression in an IkappaB kinase beta-dependent manner. Electrophoresis mobility shift assay and ChIP assay demonstrated that p65 bound to the kappaB-like DNA sequence located at approximately -9kbp in the SerpinE2 promoter. In addition, TNF-alpha stimulated luciferase gene expression driven by the kappaB-like element in the reconstituted MEFs, but not in p65(-/-) MEFs. These results indicated that activation of NF-kappaB p65 plays an important role in TNF-alpha-induced expression of SerpinE2. |
