| First Author | Burgdorf S | Year | 2008 |
| Journal | Nat Immunol | Volume | 9 |
| Issue | 5 | Pages | 558-66 |
| PubMed ID | 18376402 | Mgi Jnum | J:134501 |
| Mgi Id | MGI:3788983 | Doi | 10.1038/ni.1601 |
| Citation | Burgdorf S, et al. (2008) Spatial and mechanistic separation of cross-presentation and endogenous antigen presentation. Nat Immunol 9(5):558-66 |
| abstractText | Antiviral or antitumor immunity requires activation of cytotoxic CD8+ T cells by dendritic cells, which present viral or tumor antigens on major histocompatibility complex (MHC) class I molecules. The intracellular mechanisms facilitating MHC class I-restricted presentation of extracellular antigens ('cross-presentation') are unclear. Here we demonstrate that cross-presentation of soluble antigen occurred in an early endosomal compartment distinct from the endoplasmic reticulum where endogenous antigen is loaded onto MHC class I. Efficient cross-presentation required endotoxin-induced, Toll-like receptor 4- and signaling molecule MyD88-dependent relocation of the transporter associated with antigen processing, essential for loading of MHC class I, to early endosomes. Transport of cross-presented antigen from endosomes to the cell surface was inhibited by primaquine, which blocks endosomal trafficking. Thus, cross-presentation is spatially and mechanistically separated from endogenous MHC class I-restricted antigen presentation and is biased toward antigens containing microbial molecular patterns. |
