| Primary Identifier | MGI:1860970 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ltb |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette flanked by loxP sites (floxed) replaced exon 3 of the gene. Mice carrying this allele were bred to mice that constitutively expressed cre-recombinase in all cells in early development (Schwenk et al., Nucleic Acids Res, 1995, 23:5080-5081). Resulting mice carrying the desired deletion were intercrossed to yield homozygous mutant mice at the desired locus. Northern blots of thymocytes and Con-A stimulated splenocytes from homozygous mutant mice showed no dectectable mRNA for the targeted gene. |
