| First Author | Warren BD | Year | 2021 |
| Journal | Am J Pathol | PubMed ID | 34126085 |
| Mgi Jnum | J:307613 | Mgi Id | MGI:6721219 |
| Doi | 10.1016/j.ajpath.2021.05.021 | Citation | Warren BD, et al. (2021) Multiple Lesions Contribute to Infertility in Males Lacking Autoimmune Regulator. Am J Pathol |
| abstractText | Male factors, including those of autoimmune origin, contribute to approximately 50% of infertility cases in humans. However, the mechanisms underlying autoimmune male infertility are poorly understood. Deficiency in autoimmune regulator (AIRE) impairs central immune tolerance because of diminished expression of self-antigens in the thymus. Humans with AIRE mutations and mice with engineered ablation of Aire develop multiorgan autoimmunity and infertility. To determine the immune targets contributing to infertility in male Aire-deficient ((-/-)) mice, we paired Aire(-/-) or wild-type (WT) males with WT females. Aire(-/-) males exhibited dramatically reduced mating frequency and fertility, hypogonadism, and reduced serum testosterone. Approximately 15% of mice exhibited lymphocytic infiltration into the testis, accompanied by atrophy, azoospermia, and reduced numbers of mitotically active germ cells; the remaining mice showed normal testicular morphology, sperm counts, and motility. However, spermatozoa from all Aire(-/-) mice were defective in their ability to fertilize WT oocytes in vitro. Lymphocytic infiltration into the epididymis, seminal vesicle, and prostate gland was evident. Aire(-/-) male mice generated autoreactive antibodies in an age-dependent manner against sperm, testis, epididymis, prostate gland, and seminal vesicle. Finally, expression of Aire was evident in the seminiferous epithelium in an age-dependent manner, as well as the prostate gland. These findings suggest that Aire-dependent central tolerance plays a critical role in maintaining male fertility by stemming autoimmunity against multiple reproductive targets. |
