| First Author | Murphy JE | Year | 2003 |
| Journal | J Immunol | Volume | 170 |
| Issue | 11 | Pages | 5697-703 |
| PubMed ID | 12759452 | Mgi Jnum | J:83467 |
| Mgi Id | MGI:2662062 | Doi | 10.4049/jimmunol.170.11.5697 |
| Citation | Murphy JE, et al. (2003) IL-1alpha, Innate Immunity, and Skin Carcinogenesis: The Effect of Constitutive Expression of IL-1alpha in Epidermis on Chemical Carcinogenesis. J Immunol 170(11):5697-703 |
| abstractText | Tumor promoters such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) are proinflammatory agents, and their mechanism of action in epithelial carcinogenesis has been linked to the release of IL-1alpha and the induction of chronic inflammation in skin. To test the role of IL-1alpha and inflammation in models of cutaneous carcinogenesis, we used our previously described FVB/N transgenic mice overexpressing 17-kDa IL-1alpha in the epidermis under the keratin 14 (K14) promoter. Strikingly, the K14/IL-1alpha mice were completely resistant to papilloma and carcinoma formation induced by a two-stage DMBA/TPA protocol, while littermate controls developed both tumor types. K14/IL-1alpha mice crossed with the highly sensitive TG.AC mice, constitutively expressing mutant Ha-Ras, also failed to develop papillomas or carcinomas. When the K14/IL-1alpha transgene was bred onto a recombinase-activating gene-2-deficient background, the resistance persisted, indicating that innate, but not acquired, mechanisms may be involved in the resistance to the initiation/promotion model. As an alternative approach, a complete carcinogenesis protocol using repetitive application of DMBA alone was applied. Surprisingly, although the IL-1alpha mice still did not develop papillomas, they did develop carcinomas de novo at an accelerated rate compared with controls. We conclude that constitutive IL-1alpha expression rendered FVB mice completely resistant to carcinomas that required evolution from prior papillomas, but facilitated carcinomas that did not evolve from papillomas, as in the complete carcinogenesis protocol. Thus, the role of IL-1alpha and, by extension that of other proinflammatory factors, in epithelial carcinogenesis are more complex than previously appreciated. These mice may provide a mechanism to investigate the validity of these models of human skin tumorigenesis. |
