| First Author | Bouneaud C | Year | 2000 |
| Journal | Immunity | Volume | 13 |
| Issue | 6 | Pages | 829-40 |
| PubMed ID | 11163198 | Mgi Jnum | J:66565 |
| Mgi Id | MGI:1928626 | Doi | 10.1016/s1074-7613(00)00080-7 |
| Citation | Bouneaud C, et al. (2000) Impact of negative selection on the T cell repertoire reactive to a self-peptide: a large fraction of T cell clones escapes clonal deletion. Immunity 13(6):829-40 |
| abstractText | How negative selection shapes a polyclonal population of self-reactive T cells has been difficult to address directly because of the lack of means to isolate T cells reactive to a particular self-peptide. Here, using mice transgenic for the TCR-beta chain of a CTL clone directed against a male-specific peptide, we compared the preimmune repertoire reactive to this peptide in male and female animals. Surprisingly, in the presence of the deleting ligand, as many as 25%-40% of reactive T cells escaped clonal deletion. A correlation was found between T cell avidity, TCRalpha structures, and susceptibility to negative selection. These results suggest that numerous low-affinity self-specific T cells persist in the periphery and show that a deleting ligand can specifically narrow the structural diversity of the TCR repertoire. |
