| First Author | Alvarez Arias DA | Year | 2010 |
| Journal | Proc Natl Acad Sci U S A | Volume | 107 |
| Issue | 6 | Pages | 2556-61 |
| PubMed ID | 20133794 | Mgi Jnum | J:157540 |
| Mgi Id | MGI:4431105 | Doi | 10.1073/pnas.0913671107 |
| Citation | Arias DA, et al. (2010) Unexpected role of clathrin adaptor AP-1 in MHC-dependent positive selection of T cells. Proc Natl Acad Sci U S A 107(6):2556-61 |
| abstractText | Trafficking of transmembrane receptors to a specific intracellular compartment is conducted by adaptor molecules that bind to target motifs within the cytoplasmic domains of cargo proteins. We generated mice containing a lymphoid-specific deficiency of AP-1 using RNAi knockdown technology. Inhibition of AP-1 expression in thymocytes blocks progression from double-positive immature thymocytes, resulting in complete absence of CD4(+) single-positive thymocytes and severe reduction of CD3(+)CD8(+) single-positive thymocytes. Analysis of the contribution of AP-1 deficiency on the interaction between mature CD4(+) T cells and antigen-presenting cells revealed that AP-1 is essential to efficient immune synapse formation and associated T cell activation, suggesting a possible mechanism of AP-1 function in thymocyte development. |
